Uji Diagnosis NS1, IgG dan IgM Dengue Metode Immunokromatografi dan Elisa

Main Article Content

Suryanata Kesuma

Abstract

Dengue fever is a disease caused by the dengue virus (DENV). Detection of NS1 antigen can be useful for early confirmation of DENV infection. NS1 antigen can be detected from the first day of illness and lasts up to 9-10 days. Other supporting examinations are dengue virus IgG and IgM examinations. The purpose of this study was to review the sensitivity and specificity of the dengue virus NS1, IgG, and IgM tests through literature testing. This type of research is descriptive. The literature used is scientific journals which are the top journals searched using the google search engine and contains complete information and data related to the sensitivity and specificity of NS1, IgG, and IgM Dengue Virus examinations. Based on the review, it was found that the sensitivity of the NS1 antigen in the Rapid Immunochromatographic method was in the range of 48%-92% and specificity was 73%-100%, in the ELISA method the sensitivity range was 42%-84% and specificity was 89%-99%. In the examination of IgG and IgM antibodies using the Rapid Immunochromatography method, the sensitivity ranges from 6%-73% and specificity 36%-100%. Examination of IgG and IgM antibodies using the ELISA method obtained sensitivity in the range of 6%-58% and specificity 56%-100%. The results of the review conducted showed that the sensitivity of the NS1, IgG, and IgM tests for Dengue Virus with the Rapid Immunochromatography method was better than the ELISA method, but the specificity for the NS1, IgG, and IgM tests for Dengue Virus ELISA method was better than the Rapid Immunochromatography method. In addition, the Rapid Immunochromatography method of NS1 antigen examination showed higher accuracy than IgG and IgM antibodies in screening for dengue virus infection.

Downloads

Download data is not yet available.

Article Details

How to Cite
Kesuma, S. (2022). Uji Diagnosis NS1, IgG dan IgM Dengue Metode Immunokromatografi dan Elisa. JURNAL ANALIS LABORATORIUM MEDIK, 7(2), 72–85. https://doi.org/10.51544/jalm.v7i2.3374
Section
Artikel

References

Cucunawangsih, Lugito, N. P. H., & Kurniawan, A. (2015). Immunoglobulin G (IgG) to IgM ratio in secondary adult dengue infection using samples from early days of symptoms onset. BMC Infectious Diseases, 15(1). https://doi.org/10.1186/s12879-015-1022-9.

Granger, D., Leo, Y. S., Lee, L. K., & Theel, E. S. (2017). Serodiagnosis of dengue virus infection using commercially available antibody and NS1 antigen ELISAs. Diagnostic Microbiology and Infectious Disease, 88(2), 120–124. https://doi.org/10.1016/j.diagmicrobio.2017.03.015

Jang, W. S., Kwak, S. Y., May, W. L., Yang, D. J., Nam, J., & Lim, C. S. (2019). Comparative evaluation of three dengue duo rapid test kits to detect NS1, IgM, and IgG associated with acute dengue in children in Myanmar. PLoS ONE, 14(3). https://doi.org/10.1371/journal.pone.0213451

Liu, L. T., Chen, C. H., Tsai, C. Y., Lin, P. C., Hsu, M. C., Huang, B. Y., Wang, Y. H., & Tsai, J. J. (2020). Evaluation of rapid diagnostic tests to detect dengue virus infections in Taiwan. PLoS ONE, 15(9 September). https://doi.org/10.1371/journal.pone.0239710

Liu, L. T., Dalipanda, T., Jagilly, R., Wang, Y. H., Lin, P. C., Tsai, C. Y., Lai, W. ter, & Tsai, J. J. (2018). Comparison of two rapid diagnostic tests during a large dengue virus serotype 3 outbreak in the Solomon Islands in 2013. PLoS ONE, 13(8). https://doi.org/10.1371/journal.pone.0202304

Megariani, M., Mariko, R., Alkamar, A., & Putra, A. E. (2016). Uji Diagnostik Pemeriksaan Antigen Nonstruktural 1 untuk Deteksi Dini Infeksi Virus Dengue pada Anak. Sari Pediatri, 16(2), 121. https://doi.org/10.14238/sp16.2.2014.121-7

Nugraheni, E., Sulistyowati, I., Mikrobiologi Fakultas Kedokteran dan Ilmu Kesehatan Universitas Bengkulu, B., & Anatomi Fakultas Kedokteran dan Imu Kesehatan Universitas Bengkulu, B. (2016). Diagnosis Molekuler Virus Dengue. In JK Unila | (Vol. 1).

Pal, S., Dauner, A. L., Mitra, I., Forshey, B. M., Garcia, P., Morrison, A. C., Halsey, E. S., Kochel, T. J., & Wu, S. J. L. (2014). Evaluation of dengue ns1 antigen rapid tests and elisa kits using clinical samples. PLoS ONE, 9(11). https://doi.org/10.1371/journal.pone.0113411

Pal, S., Dauner, A. L., Valks, A., Forshey, B. M., Long, K. C., Thaisomboonsuk, B., Sierra, G., Picos, V., Talmage, S., Morrison, A. C., Halsey, E. S., Comach, G., Yasuda, C., Loeffelholz, M., Jarman, R. G., Fernandez, S., An, U. S., Kochel, T. J., Jasper, L. E., & Wu, S. J. L. (2015). Multicountry prospective clinical evaluation of two enzyme-linked immunosorbent assays and two rapid diagnostic tests for diagnosing dengue fever. Journal of Clinical Microbiology, 53(4), 1092–1102. https://doi.org/10.1128/JCM.03042-14

Palomares-Reyes, C., Silva-Caso, W., del Valle, L. J., Aguilar-Luis, M. A., Weilg, C., Martins-Luna, J., Viñas-Ospino, A., Stimmler, L., Mallqui Espinoza, N., Aquino Ortega, R., Espinoza Espíritu, W., Misaico, E., & del Valle-Mendoza, J. (2019). Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG. International Journal of Infectious Diseases, 81, 31–37. https://doi.org/10.1016/j.ijid.2019.01.022

Santoso, M. S., Yohan, B., Denis, D., Hayati, R. F., Haryanto, S., Trianty, L., Noviyanti, R., Hibberd, M. L., & Sasmono, R. T. (2020). Diagnostic accuracy of 5 different brands of dengue virus non-structural protein 1 (NS1) antigen rapid diagnostic tests (RDT) in Indonesia. Diagnostic Microbiology and Infectious Disease, 98(2). https://doi.org/10.1016/j.diagmicrobio.2020.115116

Shenoy, B., Menon, A., & Biradar, S. (2014). Diagnostic utility of dengue NS1 antigen. Pediatric Infectious Disease, 6(3), 110–113. https://doi.org/10.1016/j.pid.2014.08.002

Siti Maimunah, O. (2018). HUBUNGAN HASIL PEMERIKSAAN ANTIGEN NON-STRUKTURAL 1 (Ag NS1) DENGAN DIAGNOSIS PENYAKIT INFEKSI DENGUE DI RS URIP SUMOHARJO BANDARLAMPUNG.

Situmorang, I. M., & Effrata, N. P. (2022). IDENTIFIKASI DAN GAMBARAN INDEKS KEPADATAN LARVA AEDES AEGYPTI DI SEKOLAH TINGGI ILMU KESEHATAN YANG ADA DI BEKASI TAHUN 2021. JURNAL ANALIS LABORATORIUM MEDIK, 7(1), 35–41. https://doi.org/10.51544/jalm.v7i1.2836

Tuan, N. M., Nhan, H. T., van Vinh Chau, N., Hung, N. T., Tuan, H. M., van Tram, T., le Da Ha, N., Loi, P., Quang, H. K., Kien, D. T. H., Hubbard, S., Chau, T. N. B., Wills, B., Wolbers, M., & Simmons, C. P. (2015). Sensitivity and Specificity of a Novel Classifier for the Early Diagnosis of Dengue. PLoS Neglected Tropical Diseases, 9(4). https://doi.org/10.1371/journal.pntd.0003638

Zuardi, M., Herman, R., Litbang, B. P., Bumbu, T., Lokalitbang Gunung Tinggi, J., Litbang Biomedis Aceh, L., Sultan Iskandar Muda Lrg Tgk Dilangga No, J., Besar, A., & Biomedis dan Teknologi Dasar Kesehatan, P. (2016). Medical Laboratory Technology Journal KESESUAIAN HASIL PEMERIKSAAN RT PCR, RDT NS1, DAN RDT IgM PASIEN PENYAKIT DENGUE. Medical Laboratory Technology Journal, 2(2), 56–60. http://ejurnal-analiskesehatan.web.id.