The Effectiveness of PRP on Reducing Blood Glucose Levels in Diabetic Mice
Main Article Content
Abstract
PRP contains growth factors that have the potential to repair tissue damage, such as pancreatic damage in diabetes mellitus. Pancreas damage in diabetes mellitus is characterized by hyperglycemia. This study aimed to determine the effectiveness of PRP in reducing blood glucose levels in diabetic mice. Male Swiss Webster mice divided into 5 groups (normal group, DM group, PRP1 group, PRP2 group and PRP3 group). The DM, PRP1, PRP2 and PRP3 groups were given streptozotocin 45 mg/kgBW to induce diabetes mellitus. Mice in the PRP1, PRP2, and PRP3 groups that have indicated diabetes mellitus with glucose levels > 200 mg/dL will be given PRP with a dose of 0.1 mL; 0.3 mL; 0.5 mL intraperitoneally for 4 weeks. Data on blood glucose levels were analyzed using one-way ANOVA test and LSD test. The use of PRP in various doses, namely 0.1 mL, 0.3 mL, 0.5 mL, can reduce blood glucose levels in the PRP1 group (195.8 ± 14.04 mg/dl), PRP2 group(176.6 ± 9.37 mg/dl), PRP3 group (121 ± 5.52 mg/dl) compared to the DM group (392.6 ± 18.09 mg/dl). Dose of PRP of 0.5 mL gave a better effect on pancreatic tissue repair than the PRP1 and PRP2 groups, which were characterized by glucose levels that were close to the normal group (106.8 ± 10.61 mg/dl). Based on the results of the study, PRP 0.5 mL is effective in repairing pancreatic tissue which is characterized by a decrease in blood glucose levels in diabetic mice.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Syarat yang harus dipenuhi oleh Penulis sebagai berikut:
Â
- Penulis menyimpan hak cipta dan memberikan jurnal hak penerbitan pertama naskah secara simultan dengan lisensi di bawah Creative Commons Attribution License yang mengizinkan orang lain untuk berbagi pekerjaan dengan sebuah pernyataan kepenulisan pekerjaan dan penerbitan awal di jurnal ini.
- Penulis bisa memasukkan ke dalam penyusunan kontraktual tambahan terpisah untuk distribusi non ekslusif versi kaya terbitan jurnal (contoh: mempostingnya ke repositori institusional atau menerbitkannya dalam sebuah buku), dengan pengakuan penerbitan awalnya di jurnal ini.
- Penulis diizinkan dan didorong untuk mem-posting karya mereka online (contoh: di repositori institusional atau di website mereka) sebelum dan selama proses penyerahan, karena dapat mengarahkan ke pertukaran produktif, seperti halnya sitiran yang lebih awal dan lebih hebat dari karya yang diterbitkan. (Lihat Efek Akses Terbuka).
References
Ahmed, M., A. Sherif, Reffat, A. Hassan, & F. Eskander. 2017. Platelet-Rich Plasma for the Treatment of Clean Diabetic Foot Ulcers. Annals of Vascular Surgery 38: 206–11.
Aji, N. P., Bachri, M. S., Nurkhasanah. 2019. Efek Kombinasi Ektrak Etanol Herba Sambiloto (Andrographis paniculata) Dan Daun Kelor (Moringa oleifera) Terhadap Kadar Ureum dan Kreatinin pada tikus wistar yang Diinduksi Streptozotocin. Jurnal AKademi Farmasi Prayoga 4 (1):34-41.
Cozma CN, Raducu L, Jecan CR. 2016. Platelet Rich Plasma- mechanism of action and clinical applications. J Clin Investig Surg. 1(2):41–6.
Dewi IP, Dharma S, Marlina M. 2016. Pengaruh Pemberian Fibroblast Growth Factor (FGF) dari Telur Ayam Terfertilisasi Terhadap Kadar Glukosa Darah Mencit Hiperglikemia. J Sains Farm Klin. 3(1):1.
El Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA. 2017. Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab. 08 (03).
Hadi, R. S., Kusumah, I., Sandra, Y. 2019. Pengaruh Platelet-Rich Plasma (PRP) Terhadap Proliferasi dan Viabilitas Human Dermal Fibroblast (HDF) dalam Konsentrasi Glukosa Tinggi. Jurnal Biologi Indonesia 15(2): 213-217.
International Diabetes Federation. 2021. IDF Diabetes Atlas, 10th edn. Brussels,
Belgium. http://www.diabetesatlas.org
Irianto, K.A., Bakri, A.H., Kloping, N.A., 2021. Platelet Rich Plsma Injection for Soft Tissue Musculoskeletal Pain. Malays Orthop J 15(2):96-100.
Munjiati, N.E., Sulistiyowati, R., Kurniawan. 2021. Pengaruh Pemberian Streptozotocin Dosis Tunggal Terhadap Kadar Glukosa Tikus Wistar (Rattus norvegicus). The journal of medical laboratory 9(1):62-67.
Mutiarahmi, C., Hartady, T., Lesmana, R. 2021. Kajian Pustaka: Penggunaan Mencit Sebagai Hewan Coba di Laboratorium yang Mengacu pada Prinsip Kesejahteraan Hewan. Indonesia Medicus Veterinus. 134-145. doi:10.19087/imv.2021.10.1.134
Pavlovic V, Ciric M, Jovanovic V, Stojanovic P. 2016. Platelet Rich Plasma: A short overview of certain bioactive components. Open Med. 11(1):242–7.
Prawitasari DS. Diabetes Melitus dan Antioksidan. 2019. KELUWIH J Kesehat dan Kedokt.1(1):48–52.
Rizal DM, Puspitasari I, Yuliandari A. 2020. Protective Effect of PRP against Testicular Oxidative Stress on D-galactose Induced Male Rats. AIP Conf Proc. 2020;2260 (September)
Saputra, N.T., Suartha, I.N., Dharmayudha, A. A. G. O., 2018. Agen Diabetagonik Streptozotocin untuk Membuat Tikus Putih Jantan Diabetes Mellitus. Buletin Veteriner Udayana 10(2): 116-121.
Siahaan, M.A., Aruan, D.G. 2022. Analysis Of Blood Glucose Levels In People With Type II Diabetes Mellitus (DM) Aged 50-60 Years With Glucometer Method At Islamic Malahayati Medan Hospital Year 2022. Jurnal Analis Laboratorium Medik, 7(1):10-14.
Simarmata HP, Saragih RNL, Pasaribu P, Sitorus JP, Chiuman L. 2021. Efektivitas PRP Terhadap Perubahan Profil Lipid Darah Tikus Putih Jantan (Rattus norvegicus) Diabetes Melitus yang Diinduksi Streptozotocin. Biospecies.14(1):10–7.
Yuliana, T.P. 2021. Pemberian Ramuan Pengobat Tradisional Kaliputih Terhadap Kadar Gula Darah Tikus Diabetes. Jurnal Famasi Klinis dan Sains Bahan Alam 1(1): 7-15.
Zarin, M., Karbalaei, N., Keshtgara, S., Nematia, M. 2019. Platelet-rich plasma improves impaired glucose hemostasis, disrupted insulin secretion, and pancreatic oxidative stress in streptozotocin-induced diabetic rat. Growth Factors 37(5-6):226-237