Uji Efektivitas Daya Hambat Ekstrak Etanol Daun Benalu Belimbing Macrosolen cochinchinensis (Lour) V.Tiegh Terhadap Bakteri Staphylacoccus aureus
Keywords:
Antibakteri, Ekstrak Etanol, Daun Benalu Belimbing, Staphylococus aureus.Abstract
Benalu Belimbing is traditionally used to treat cancer, stop bleeding in wounds and to treat wounds as well as anti-bacterial and skin diseases. Staphylococcus aureus bacteria are bacteria that are normal flora found on the skin, nose and respiratory tract, diseases that appear such as acne, boils, ulcers, wounds and pneumonia. This research was carried out with the aim of determining the antibacterial activity of ethanol extract of Benalu Belimbing leaves (Macrosolen cochinchinensis (Lour) V.Tiegh) with concentrations of 20%, 40%, 60% and 80% against the growth of Staphylococcus aureus bacteria. This research method uses an experimental method, namely to determine the antibacterial activity of ethanol extract of Benalu Belimbing leaves (Macrosolen cochinchinensis (Lour) V.Tiegh) on the growth of Staphylococcus aureus bacteria. This research used 96% ethanol solvent. Testing was carried out in several stages including collecting materials, preparing simplicia, making ethanol extract from mistletoe starfruit leaves by maceration and testing antibacterial activity against Staphylococcus aureus bacteria. Based on the results of phytochemical screening tests, the thick extract of mistletoe starfruit leaves contains flavonoid, saponin, steroid/triterpenoid and tannin compounds. The results of the anti-bacterial activity test of the thick extract of mistletoe star fruit leaves against Staphylococcus aureus bacteria had an average inhibitory activity at concentrations of 20% (7.36 mm), 40% (8.97 mm), 60% (10.48 mm), and 80% (11.81 mm). As a comparison, Cloramfenicol 30 μg was used to produce an inhibitory power of 21.94 mm and the best results obtained were at a concentration of 80% which had an inhibitory power of 11.81 mm.
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References
Adnan, (2017), Laporan Teknis Sub Tolok Ukur Pengembangan Senyawa Potensial antikanker dari Taxus sumatrana dan Benalu Kopi, Puslit Kimia LIPI, Serpong.
Artanti, N., Firmansyah, T., and Darmawan, A. (2012). Bioactivies Evaluation of Indonesian Mistletos (Dendrophthoe pentandra (L.) Miq.) Leaves Extracts. Journal of Applied Pharmaceutical Science, 02(01): 24-27.
Anita, A., Khotimah, S., & Yanti, A.H. (2014). Aktivitas Antibakteri Ekstrak Daun Benalu Jambu Air (Dendrophthoe Pentandra (L.) Miq Terhadap Pertumbuhan Salmoela Typhy.Protobium. 3(2):266-272.
Akiyama, H., Fujii K., Yamaaki O.., Oono T.., dan Iwatsuki K., (2001). Antibacterial Action of Several Tannin against Staphyloccoccus aureus. Journal of Antimicrobial Chemotherapy. 48 : 487 – 491.
Cavalieri, S.J., I.D. Rankin, R.J. Harbeck. R.S. Sautter., Y.S. Mc Carter., S.E. Sharp., J.H., Ortez., dan C.A Spiegel. (2015). Manual of Antimicrobial Susceptibility Testing. American Society for Microbiology, USA.
Darmawan, A., dan Nila, A. (2019). Aktivitas Antioksidan Dan Bioaktivitas In Vitro Ekstrak Air dan Ekstrak Etanol Daun Dan Ranting Benalu Macrosolen Cochinchinensis (Lour.) van Tiegh. Pada Inang Nangka (Artocarpus Heterophyllus). Pusat Penelitian Kimia-LIPI. Kawasan PUSPIPTEK Serpong, Tangerang, Banten.
Depkes RI. (1995). Materia Medikal Indonsia. Jilid IV. Cetakan Keenam. Jakarta: Departemen Kesehatan Republik Indonesia. Halaman 297-303 dan 334-337.
Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Cetakan Pertama. Jakarta:Departemen Kesehatan Republik Indonesia. Hal.10-11.
Ditjen POM. (1979). Farmakope Indonesia. Edisi Ketiga. Jakarta: Departemen Kesehatan RI. Hal 9-649.
Droge, W. Free Radicals In The Physiological Control Of Cell Function. Physiol Rev. 2002, 82, 47-95.
Fidrianny, I., Darmawati, A., Dan Sukrasno. (2014). Antioxidant Capacities From Different Polarities Extracts of Cucurbitaceae Leaves Using Frap, DPPH Assays And Correlation With Fenolic, Flavonoid, Carotenoid Content. International journal of pharmacy and pharmaceutical Science. 6(2): 858- 862.
Gandjar, I.G., dan Rohman, A. (2017). Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar. Hal. 222, 252-256.
Gordon. (1990). The mehanism of antioxidant in vitro. Dalam: Hudson, B.J.F. (Ed.). Food Antioxidant. Elservier Applied Science, London and New York.
Gupta, A.D. & Rajpurohit, D. (2011). Antioxidant and Antimicrobial Activity of Nutmeg (Myristica fragrans). In Preedy, V.R., Watson, R.R. & Patel, V.B. (eds). Nuts and Seeds in Health and Disease Prevention. Page 831 – 838.
